3VVN
Crystal structure of MATE in the straight conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-21 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.979, 0.900, 0.910 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.889, 60.100, 68.127 |
| Unit cell angles | 90.00, 90.27, 90.00 |
Refinement procedure
| Resolution | 43.331 - 2.398 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.753 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.398 | 6.510 | 2.400 |
| Rmerge | 0.116 | 0.043 | 0.370 |
| Number of reflections | 24440 | ||
| <I/σ(I)> | 5.7 | ||
| Completeness [%] | 98.1 | 99.4 | 94.7 |
| Redundancy | 5.4 | 6.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | lipdic cubic phase | 6.5 | 293 | 32% PEG 400, 50mM MES-NaOH, 20mM CaCl2, 100mM NaSCN, pH 6.5, lipdic cubic phase, temperature 293K |
