3VUW
Crystal structure of A20 ZF7 in complex with linear ubiquitin, form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-21 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.20000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.173, 51.991, 52.180 |
| Unit cell angles | 70.08, 80.25, 78.05 |
Refinement procedure
| Resolution | 41.501 - 1.950 |
| R-factor | 0.1803 |
| Rwork | 0.178 |
| R-free | 0.22210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.141 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 5.290 | 1.950 |
| Rmerge | 0.057 | 0.044 | 0.270 |
| Number of reflections | 26390 | ||
| <I/σ(I)> | 18 | ||
| Completeness [%] | 96.2 | 96.4 | 78.7 |
| Redundancy | 10.5 | 9.6 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 10mM Tris-HCl, 150mM NaCl, pH 8.0, vapor diffusion, hanging drop, temperature 293K |
