3VUU
Crystal structure of the merozoite surface protein MSPDBL2 from P. falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0163 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.625, 101.499, 38.365 |
| Unit cell angles | 90.00, 101.59, 90.00 |
Refinement procedure
| Resolution | 37.583 - 2.093 |
| R-factor | 0.1851 |
| Rwork | 0.183 |
| R-free | 0.23120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wau |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.926 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.160 |
| High resolution limit [Å] | 2.090 | 4.500 | 2.090 |
| Number of reflections | 16184 | ||
| Completeness [%] | 99.7 | 100 | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 300 | 20% PEG3350, 0.2M sodium thiocyanate, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
