3VUS
Escherichia coli PgaB N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-07-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.559, 53.114, 144.170 |
| Unit cell angles | 90.00, 95.25, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.650 |
| R-factor | 0.20587 |
| Rwork | 0.205 |
| R-free | 0.25633 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.294 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.049 | 0.375 |
| Number of reflections | 69364 | |
| Completeness [%] | 96.7 | 83.7 |
| Redundancy | 4.9 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.18M Sodium acetate, 0.1M Bis-Tris, 16%(w/v) PEG3350, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.18M Sodium acetate, 0.1M Bis-Tris, 16%(w/v) PEG3350, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
