3VUF
Crystal Structure of Rice Granule bound Starch Synthase I Catalytic Domain in Complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-12-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 153.368, 153.368, 153.368 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.190 - 3.000 |
| R-factor | 0.22357 |
| Rwork | 0.221 |
| R-free | 0.27088 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vue |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.619 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.067 | 0.669 |
| Number of reflections | 12909 | |
| <I/σ(I)> | 24.5 | 4.4 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 14 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.5M Lithium Sulfate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
