3VUE
Crystal Structure of Rice Granule bound Starch Synthase I Catalytic Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2009-01-01 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 4 3 2 |
Unit cell lengths | 152.904, 152.904, 152.904 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.700 |
R-factor | 0.22077 |
Rwork | 0.218 |
R-free | 0.26707 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3guh |
RMSD bond length | 0.015 |
RMSD bond angle | 1.602 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.046 | 0.370 |
Number of reflections | 17202 | |
<I/σ(I)> | 27.5 | 7 |
Completeness [%] | 99.3 | 99.9 |
Redundancy | 13.5 | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.5M Lithium Sulfate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |