3VTZ
Structure of Thermoplasma volcanium aldohexose dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 |
| Unit cell lengths | 110.018, 110.018, 77.172 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.790 - 2.300 |
| R-factor | 0.17007 |
| Rwork | 0.167 |
| R-free | 0.23201 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dtd |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.042 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.084 | 0.509 |
| Number of reflections | 43989 | |
| <I/σ(I)> | 24.1 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris pH 8.5, 0.2M Li2SO4, 28% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
