3VTO
The crystal structure of the C-terminal domain of Mu phage central spike
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-02 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.403, 152.399, 53.062 |
| Unit cell angles | 90.00, 91.01, 90.00 |
Refinement procedure
| Resolution | 19.520 - 1.440 |
| R-factor | 0.16391 |
| Rwork | 0.162 |
| R-free | 0.19458 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.636 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.460 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Number of reflections | 114876 | |
| Completeness [%] | 99.7 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 16% PEG 20000, 0.1M MES, 0.15M sodium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
