3VT8

Crystal structures of rat VDR-LBD with W282R mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Collection date	2008-02-14
Wavelength(s)	0.97800
Spacegroup name	C 1 2 1
Unit cell lengths	155.068, 42.052, 41.868
Unit cell angles	90.00, 95.62, 90.00

Refinement procedure

Resolution	38.260 - 2.100
R-factor	0.189
Rwork	0.189
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zlc
RMSD bond length	0.004
RMSD bond angle	0.700
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	15047

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	MOPS-Na, di-Ammonium Citrate, PEG 4000, 2-propanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K