3VRN

Crystal structure of the tyrosine kinase binding domain of Cbl-c

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	90
Detector technology	CCD
Collection date	2007-06-25
Detector	Bruker DIP-6040
Wavelength(s)	0.900
Spacegroup name	C 2 2 21
Unit cell lengths	93.223, 107.617, 54.773
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.240 - 1.640
R-factor	0.1834
Rwork	0.182
R-free	0.21770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cbl
RMSD bond length	0.022
RMSD bond angle	1.849
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.670
High resolution limit [Å]	1.640	4.450	1.640
Rmerge	0.054	0.027	0.446
Number of reflections	34203
<I/σ(I)>	27.992	50.879	3.452
Completeness [%]	100.0	99.8	100
Redundancy	4.3	4.1	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	16% PEG3350, 0.1M ammonium formate, 0.2M NDSB-201, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K