3VRG
The crystal structure of hemoglobin from woolly mammoth in the met form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-01-24 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.398, 61.473, 53.488 |
| Unit cell angles | 90.00, 110.08, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.18758 |
| Rwork | 0.186 |
| R-free | 0.22047 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dn3 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.059 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.047 | 0.126 |
| Number of reflections | 53178 | |
| Completeness [%] | 96.7 | 96.2 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Ammonium sulfate, Tris-HCl, pH 6.5, temperature 293K, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
