3VQX
Crystal structure of the catalytic domain of pyrrolysyl-tRNA synthetase in triclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.798, 69.422, 83.326 |
| Unit cell angles | 106.92, 91.41, 113.83 |
Refinement procedure
| Resolution | 48.150 - 2.300 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vqw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 45278 | |
| Completeness [%] | 83.9 | 64.3 |
| Redundancy | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | Sodium Cacodylate, Sodium/Potassium tartrate, Ammonium Sulfate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
