3VPV

Crystal Structure of Pseudomonas aeruginosa Tsi2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	95
Detector technology	CCD
Collection date	2011-11-14
Detector	MAR CCD 165 mm
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	28.380, 34.920, 38.490
Unit cell angles	110.37, 95.98, 95.61

Refinement procedure

Resolution	19.794 - 1.800
R-factor	0.1862
Rwork	0.185
R-free	0.21600
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.742
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX (1.7_650)
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.794	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.022	0.047
Number of reflections	12200
<I/σ(I)>	36.5	16
Completeness [%]	99.4	95.7
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	10% PEG 3350, 0.1mM sodium acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K