3VPB
ArgX from Sulfolobus tokodaii complexed with LysW/Glu/ADP/Mg/Zn/Sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-10-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.546, 113.988, 78.582 |
| Unit cell angles | 90.00, 102.43, 90.00 |
Refinement procedure
| Resolution | 28.910 - 1.800 |
| R-factor | 0.18931 |
| Rwork | 0.187 |
| R-free | 0.23505 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uc8 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.454 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 106366 | |
| Completeness [%] | 99.7 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 13% PEG-MME 5000, 0.1M Ammonium sulfate, 0.1M MES-NaOH (pH6.0), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
