3VP9
Crystal structure of the N-terminal domain of the yeast general corepressor Tup1p mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.102, 41.461, 103.889 |
| Unit cell angles | 90.00, 95.35, 90.00 |
Refinement procedure
| Resolution | 33.292 - 1.799 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.25750 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.071 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 409.000 | 1.830 |
| High resolution limit [Å] | 1.799 | 1.800 |
| Number of reflections | 20399 | |
| Completeness [%] | 91.4 | 91.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 14%(v/v) 1,4-dioxane, 0.1M MES monohydrate, 1.6M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, temperature 293K |
