3VOU
The crystal structure of NaK-NavSulP chimera channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 104.227, 104.227, 104.444 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.910 - 3.200 |
| R-factor | 0.2933 |
| Rwork | 0.292 |
| R-free | 0.32192 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ahy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.019 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.910 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.077 | 0.654 |
| Number of reflections | 10367 | |
| <I/σ(I)> | 41.1 | 4.3 |
| Completeness [%] | 92.6 | 83.1 |
| Redundancy | 8.3 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 19-21% polyethylene glycol monomethyl ether 550, 0.05M magnesium nitrate, 0.1M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
