3VOQ
Crystal structure of the pleckstrin homology domain of human Sin1, a TORC2 subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-02 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.293, 79.293, 123.599 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.530 - 2.000 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.22390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ulb |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.461 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.530 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.105 | 0.911 |
| Number of reflections | 27409 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.6 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M Bis-Tris, 1.26M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
