3VOK
X-ray Crystal Structure of Wild Type HrtR in the Apo Form with the Target DNA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-23 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 97.870, 97.870, 108.695 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.870 - 2.000 |
| R-factor | 0.2037 |
| Rwork | 0.202 |
| R-free | 0.23750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qpi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.106 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.079 | 0.513 |
| Number of reflections | 21224 | |
| <I/σ(I)> | 41.584 | 6.622 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 21.5 | 21.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | 1.2M sodium citrate, 0.1M PIPES, pH 7.3, vapor diffusion, sitting drop, temperature 293K |
