3VOG
Catalytic domain of the cellobiohydrolase, CcCel6A, from Coprinopsis cinerea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-22 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.963, 72.493, 104.945 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.260 - 1.450 |
R-factor | 0.1714 |
Rwork | 0.170 |
R-free | 0.19840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bvw |
RMSD bond length | 0.010 |
RMSD bond angle | 1.336 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.053 | 0.356 |
Number of reflections | 61610 | |
<I/σ(I)> | 41.4 | 5.1 |
Completeness [%] | 97.6 | 92.7 |
Redundancy | 9.97 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 8000, 0.1M HEPES-KOH, 0.1M magnesium acetate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |