3VNY

Crystal structure of beta-glucuronidase from Acidobacterium capsulatum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	CCD
Collection date	2010-01-29
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	I 41 2 2
Unit cell lengths	101.126, 101.126, 217.881
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.580 - 1.500
R-factor	0.17264
Rwork	0.172
R-free	0.18987
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.066
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.684	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.062	0.392
Number of reflections	90146
<I/σ(I)>	47	6.8
Completeness [%]	100.0	100
Redundancy	14.6	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.0M sodium phosphate monobasic monohydrate/potassium phosphate dibasic (0.5/9.5 [v/v]), VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP		293	2.0M sodium phosphate monobasic monohydrate/potassium phosphate dibasic (0.5/9.5 [v/v]), VAPOR DIFFUSION, SITTING DROP, temperature 293K