3VNE

Structure of the ebolavirus protein VP24 from Sudan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 31 2 1
Unit cell lengths	61.052, 61.052, 130.352
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.569 - 2.000
R-factor	0.2241
Rwork	0.220
R-free	0.26280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.106
Data reduction software	d*TREK
Data scaling software	d*TREK
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.600
High resolution limit [Å]	2.000	2.000
Rmerge	0.068
Number of reflections	19519
<I/σ(I)>	8.4
Completeness [%]	99.6	99.6
Redundancy	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	295	0.1M HEPES, 0.1M MgCl, 8% PEG 550mme, pH 7.5, vapor diffusion, temperature 295K