3VN5
Crystal structure of Aquifex aeolicus RNase H3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-10 |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 48.943, 48.943, 293.217 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.870 - 1.980 |
| R-factor | 0.20603 |
| Rwork | 0.204 |
| R-free | 0.23844 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d0a |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.049 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (refmac_5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.010 |
| High resolution limit [Å] | 1.980 | 5.370 | 1.980 |
| Rmerge | 0.104 | 0.060 | 0.289 |
| Number of reflections | 29839 | ||
| <I/σ(I)> | 6.1 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 21.3 | 18.7 | 21.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | 0.1M citrate buffer (pH 4.0), 0.2M sodium citrate, 20% w/v polyethylene glycol (PEG) 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
