3VN3
Fungal antifreeze protein exerts hyperactivity by constructing an inequable beta-helix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.7000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.890, 41.630, 70.250 |
| Unit cell angles | 81.53, 83.13, 62.43 |
Refinement procedure
| Resolution | 34.410 - 0.950 |
| R-factor | 0.11302 |
| Rwork | 0.112 |
| R-free | 0.12948 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.851 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.410 | 1.000 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.060 | 0.396 |
| Number of reflections | 232289 | |
| <I/σ(I)> | 19.9 | 2 |
| Completeness [%] | 95.5 | 92 |
| Redundancy | 8.6 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 0.1M Bis-Tris, 1.0M ammonium sulfate, 0.1M sodium chloride, pH 6.0, VAPOR DIFFUSION, temperature 293K |
