3VMQ

Crystal structure of Staphylococcus aureus membrane-bound transglycosylase: Apoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-28
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	66.683, 67.406, 152.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.079 - 2.518
R-factor	0.2027
Rwork	0.200
R-free	0.25670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hzs 3FWM 3fwl
RMSD bond length	0.013
RMSD bond angle	0.841
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.079	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	23658
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	100mM MgCl2, 100mM HEPES, 25% PEG400, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K