3VM8

Crystal structure of the human APOBEC3C having HIV-1 Vif-binding interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-05-27
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 61
Unit cell lengths	104.419, 104.419, 74.502
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	90.430 - 3.000
R-factor	0.2315
Rwork	0.228
R-free	0.30230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ir2
RMSD bond length	0.006
RMSD bond angle	0.992
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	1000.000	1000.000	3.050
High resolution limit [Å]	3.000	8.140	3.000
Rmerge	0.108	0.050	0.522
Number of reflections	9105
<I/σ(I)>	13.9
Completeness [%]	97.0	96.6	100
Redundancy	10.2	10.1	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	Bicine, PEG 6000, L-Arginine HCl, pH 9.0, vapor diffusion, hanging drop, temperature 293K