3VM7
Structure of an Alpha-Amylase from Malbranchea cinnamomea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-06-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.900, 84.940, 56.410 |
| Unit cell angles | 90.00, 105.61, 90.00 |
Refinement procedure
| Resolution | 49.990 - 2.250 |
| R-factor | 0.14687 |
| Rwork | 0.143 |
| R-free | 0.19080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.419 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.520 | 2.380 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.065 | |
| Number of reflections | 22344 | |
| <I/σ(I)> | 0.078 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 0.2M ammonium acetate, 0.1M sodium acetate trihydrate pH 4.6, 30% w v-1 PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
