3VLK
Crystal Structure Analysis of the Ser305Ala variant of KatG from Haloarcula marismortui
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-17 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 321.242, 76.665, 74.712 |
| Unit cell angles | 90.00, 99.54, 90.00 |
Refinement procedure
| Resolution | 49.970 - 2.000 |
| R-factor | 0.242 |
| Rwork | 0.242 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.138 | 0.696 |
| Number of reflections | 120317 | |
| <I/σ(I)> | 7 | 4.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 2.92-3.0M ammonium sulfate, 0.5M potassium chloride, 20mM sodium phosphate, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
