3VLE
Crystal structure of yeast proteasome interacting protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-03 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.801, 91.893, 78.949 |
| Unit cell angles | 90.00, 113.06, 90.00 |
Refinement procedure
| Resolution | 72.640 - 2.410 |
| R-factor | 0.20937 |
| Rwork | 0.206 |
| R-free | 0.27397 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vld |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.951 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.640 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.064 | 0.319 |
| Number of reflections | 40079 | |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 100.0 | 99.4 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 8% PEG 3000, 0.2M potassium phosphate, 0.1M Tris-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
