3VL1
Crystal structure of yeast Rpn14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-25 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.491, 141.443, 50.491 |
| Unit cell angles | 90.00, 95.67, 90.00 |
Refinement procedure
| Resolution | 24.470 - 1.600 |
| R-factor | 0.158 |
| Rwork | 0.154 |
| R-free | 0.23451 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3acp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.940 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.620 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.048 | 0.528 |
| Number of reflections | 90359 | |
| <I/σ(I)> | 19.5 | |
| Completeness [%] | 97.2 | 97.2 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1M NaCitrate pH4.5, 0.2M MgCl2, 10%(w/v) PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
