3VK9

Crystal structure of delta-class glutathione transferase from silkmoth

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	128.393, 162.184, 86.791
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.449 - 2.001
R-factor	0.1913
Rwork	0.189
R-free	0.24490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.034
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	32.100
High resolution limit [Å]	2.001
Number of reflections	60864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2M Sodium acetate trihydrate, 0.1M Sodium cacodylate trihydrate, 30% PEG8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K