3VK6
Crystal structure of a phosphotyrosine binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 64.657, 64.657, 121.035 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.228 - 1.900 |
| R-factor | 0.2216 |
| Rwork | 0.219 |
| R-free | 0.24240 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.172 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 21480 | |
| <I/σ(I)> | 21.3 | |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 22.8 | 22.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 200mM Li2SO4, 100mM Tris, 20% PEG 5000 MME, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
