3VJZ
Crystal structure of the DNA mimic protein DMP19
Experimental procedure
| Experimental method | MAD |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-01-28 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418, 1.0397, 1.0229, 1.0400 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.878, 65.309, 59.094 |
| Unit cell angles | 90.00, 99.70, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.1716 |
| Rwork | 0.170 |
| R-free | 0.20960 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.775 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.058 | 0.874 |
| Number of reflections | 30584 | |
| <I/σ(I)> | 47.3 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.5 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 298 | 2M Ammonium sulfate, 200mM Potassium sodium tartrate tetrahydrate, 100mM Sodium citrate tribasic dihydrate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
