3VJ7
Crystal structure of the carboxy-terminal ribonuclease domain of Colicin E5 R33Q mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-28 |
| Detector | MAC Science DIP-2030 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.688, 49.450, 64.619 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.270 - 2.300 |
| R-factor | 0.23888 |
| Rwork | 0.238 |
| R-free | 0.25730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2djh |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.833 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 4574 | |
| Completeness [%] | 99.8 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 2M ammonium sulfate, 20mM TrisCl, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
