3VIJ
Crystal structure of beta-glucosidase from termite Neotermes koshunensis in complex with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-09 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.90000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.948, 68.403, 75.052 |
| Unit cell angles | 90.00, 95.48, 90.00 |
Refinement procedure
| Resolution | 26.400 - 1.030 |
| R-factor | 0.12079 |
| Rwork | 0.120 |
| R-free | 0.13536 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ahz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.399 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.070 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Rmerge | 0.051 | 0.426 |
| Number of reflections | 229380 | |
| <I/σ(I)> | 30.8 | 3.4 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 4.9 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M Bis-Tris, 18-21%(w/v) PEG 3350, 0.1-0.25M MgCl2, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
