3VIH
Crystal structure of beta-glucosidase from termite Neotermes koshunensis in complex with glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.80000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.155, 68.526, 75.469 |
| Unit cell angles | 90.00, 95.58, 90.00 |
Refinement procedure
| Resolution | 28.000 - 1.030 |
| R-factor | 0.14874 |
| Rwork | 0.148 |
| R-free | 0.16818 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ahz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.399 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.070 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Rmerge | 0.080 | 0.931 |
| Number of reflections | 224739 | |
| <I/σ(I)> | 23.3 | 2.3 |
| Completeness [%] | 96.8 | 99.8 |
| Redundancy | 5.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M Bis-Tris, 18-21%(w/v) PEG 3350, 0.1-0.25M MgCl2, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
