3VIC
Green-fluorescent variant of the non-fluorescent chromoprotein Rtms5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-18 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 150.271, 186.060, 185.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.800 - 2.200 |
| R-factor | 0.16189 |
| Rwork | 0.160 |
| R-free | 0.20447 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mou |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.022 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 117.040 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 130290 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 22% PEG 3350, 0.34M POTASSIUM IODIDE, 0.2M TRIS, PH 8.50, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293.0K |
