3VHZ
Crystal structure of the trans isomer of the L93A mutant of bacteriorhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 102.600, 102.600, 112.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| R-factor | 0.2524 |
| R-free | 0.28450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iw6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.209 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.900 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.065 | 0.273 |
| Number of reflections | 15435 | |
| <I/σ(I)> | 47.6 | 14.1 |
| Completeness [%] | 96.7 | 95.9 |
| Redundancy | 8.8 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | membrane fusion method | 5.2 | 283 | membrane fusion method, 2.3M ammonium sulfate, 0.05M Na-citrate, pH 5.2, temperature 283K |
