3VHJ

Crystal structure of the cytoplasmic domain of BfpC

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-01-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791, 0.9793, 0.9639
Spacegroup name	P 21 21 21
Unit cell lengths	42.837, 61.836, 78.271
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.930 - 1.900
R-factor	0.217
Rwork	0.217
R-free	0.22730
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.900
Number of reflections	16999
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	2.0M Ammonium sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K