3VGU
E134A mutant nucleoside diphosphate kinase derived from Halomonas sp. 593
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-05 |
| Detector | RIGAKU JUPITER 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.489, 93.149, 68.410 |
| Unit cell angles | 90.00, 103.60, 90.00 |
Refinement procedure
| Resolution | 22.980 - 2.300 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nhk |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.630 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.540 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.063 | 0.251 |
| Number of reflections | 47185 | |
| <I/σ(I)> | 16 | 2.9 |
| Completeness [%] | 93.8 | 91 |
| Redundancy | 2.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.18M CALCIUM ACETATE HYDRATE, 0.09M SODIUM CACODYLATE TRIHYDRATE, 16% PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
