3VGS
Wild-type nucleoside diphosphate kinase derived from Halomonas sp. 593
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-12 |
| Detector | ADSC QUANTUM 4r |
| Spacegroup name | H 3 |
| Unit cell lengths | 112.531, 112.531, 125.981 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.350 - 2.300 |
| R-factor | 0.265 |
| Rwork | 0.263 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nhk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.129 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.080 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.046 | 0.330 |
| Number of reflections | 25691 | |
| <I/σ(I)> | 26 | 2.4 |
| Completeness [%] | 97.3 | 92.1 |
| Redundancy | 4.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.2M AMMONIUM ACETATE, 0.1M SODIUM CITRATE TRIBASIC DEHYDRATE, 30% PEG 4000, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
