3VGL
Crystal structure of a ROK family glucokinase from Streptomyces griseus in complex with glucose and AMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2008-10-17 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.00000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 69.580, 87.730, 125.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.550 |
R-factor | 0.1786 |
Rwork | 0.177 |
R-free | 0.20240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vgm |
RMSD bond length | 0.011 |
RMSD bond angle | 1.308 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (refmac_5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.590 | |
High resolution limit [Å] | 1.550 | 6.930 | 1.550 |
Rmerge | 0.051 | 0.039 | 0.346 |
Rmeas | 0.043 | 0.445 | |
Number of reflections | 53724 | 660 | 2894 |
<I/σ(I)> | 20.14 | 38.71 | 2.68 |
Completeness [%] | 96.0 | 93.2 | 70.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 0.1M Tris-HCl, 0.75M sodium citrate, 0.2M NaCl, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |