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3VGF

Crystal structure of glycosyltrehalose trehalohydrolase (D252S) complexed with maltotriosyltrehalose

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL40B2
Synchrotron siteSPring-8
BeamlineBL40B2
Temperature [K]100
Detector technologyCCD
Collection date2001-04-23
DetectorADSC QUANTUM 4r
Wavelength(s)1.0000
Spacegroup nameP 32 2 1
Unit cell lengths78.441, 78.441, 282.277
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution68.000 - 2.300
R-factor0.1794
Rwork0.177
R-free0.21760
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1eh9
RMSD bond length0.017
RMSD bond angle1.694
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareMOLREP
Refinement softwareREFMAC
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]94.0902.380
High resolution limit [Å]2.3002.300
Rmerge0.0870.366
Number of reflections45920
<I/σ(I)>40.54.5
Completeness [%]99.999.3
Redundancy9.67.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.52771.1M sodium citrate, 0.1M HEPES, 5mM MTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

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