3VG0
Antibody Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-22 |
| Detector | MAR345 |
| Wavelength(s) | 1.54182 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.219, 39.966, 105.132 |
| Unit cell angles | 90.00, 128.06, 90.00 |
Refinement procedure
| Resolution | 37.550 - 2.270 |
| R-factor | 0.1936 |
| Rwork | 0.190 |
| R-free | 0.25950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cix chains A and D for chains L and H repectively |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.897 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.550 | 2.380 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.064 | 0.277 |
| Number of reflections | 21142 | |
| <I/σ(I)> | 11.7 | 3.3 |
| Completeness [%] | 97.2 | 82 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 281 | 0.2 M ammonium sulphate, 0.1 M sodium acetate pH4.6, 25% PEG-4000, vapor diffusion, temperature 281K |
