3VEY
glucokinase in complex with glucose and ATPgS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 80.387, 80.387, 324.334 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.030 - 2.250 |
| R-factor | 0.2157 |
| Rwork | 0.214 |
| R-free | 0.24640 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.050 |
| Refinement software | BUSTER (2.9.6) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.250 |
| Rmerge | 0.082 |
| Number of reflections | 28356 |
| Completeness [%] | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | Protein at 10 mg/ml in 25 mM HEPES pH 7.0, 0.5 mM TCEP, 0.05 M NaCl, 40 mM glucose and 1 mM activator at an 1:1 ratio with well solution of 0.2 M ammonium acetate, 0.1 M Bis-Tris pH 6.5, and 19-26% PEG-4000. 1mM ATPgS*Mg was soaked overnight in reformed crystal., VAPOR DIFFUSION, HANGING DROP |
