3VEM

Structural basis of transcriptional gene silencing mediated by Arabidopsis MOM1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 31 2 1
Unit cell lengths	85.640, 85.640, 292.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.959 - 3.200
R-factor	0.2601
Rwork	0.258
R-free	0.29320
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.263
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.6.1_353))

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	3.200
Number of reflections	21107
Completeness [%]	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.1 M Tris, 0.3 M magnesium formate dihydrate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K