3VEJ
Crystal structure of the Get5 carboxyl domain from S. cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-01-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.020, 46.340, 28.270 |
| Unit cell angles | 90.00, 96.90, 90.00 |
Refinement procedure
| Resolution | 24.839 - 1.230 |
| R-factor | 0.1782 |
| Rwork | 0.177 |
| R-free | 0.20390 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.082 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.340 | 1.260 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.058 | 0.609 |
| Number of reflections | 18288 | |
| <I/σ(I)> | 11.63 | 2.05 |
| Completeness [%] | 97.9 | 94.6 |
| Redundancy | 4.2 | 3.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 3.4 M ammonium sulfate, 0.1 M sodium citrate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
