3VDY
B. subtilis SsbB/ssDNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.53, 1.008 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 101.753, 101.753, 118.571 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.23151 |
| Rwork | 0.230 |
| R-free | 0.26177 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.442 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 15468 | |
| Completeness [%] | 97.4 | 71.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 50 mM NaAcetate, pH 5.6, 10 mM MgCl(2), 1.8 M Ammonium sulfate, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
