3VDK
Crystal structure of circumsporozoite protein aTSR domain, R32 platinum-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.07195 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 66.534, 66.534, 86.488 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.267 - 1.847 |
| R-factor | 0.18 |
| Rwork | 0.176 |
| R-free | 0.21680 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.042 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.847 | 1.847 |
| Rmerge | 0.091 | 0.643 |
| Number of reflections | 6265 | |
| <I/σ(I)> | 42.6 | 2.9 |
| Completeness [%] | 98.1 | 86.9 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 295 | 0.1 M citrate, pH 4.0, 1 M lithium chloride, 20% PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
