3VDH

Crystal structure of PbGH5A, a glycoside hydrolase family 5 enzyme from Prevotella bryantii B14

Replaces:  3L55

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-10-22
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	57.908, 85.254, 74.556
Unit cell angles	90.00, 109.15, 90.00

Refinement procedure

Resolution	26.354 - 1.620
R-factor	0.1524
Rwork	0.151
R-free	0.19930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jeq
RMSD bond length	0.010
RMSD bond angle	1.222
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.550	1.600
Number of reflections	83351
<I/σ(I)>	38.95	4.67
Completeness [%]	84.0	61.6
Redundancy	3.4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	0.2 M CALCIUM ACETATE HYDRATE, 0.1M SODIUM CACODYLATE PH 6.5, 18% PEG 8K, VAPOR DIFFUSION, HANGING DROP, temperature 295K