3VDF
Crystal Structure of C-lobe of Bovine lactoferrin Complexed with diaminopimelic acid at 1.46 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2011-11-09 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.212, 50.023, 65.206 |
Unit cell angles | 90.00, 107.00, 90.00 |
Refinement procedure
Resolution | 37.870 - 1.460 |
R-factor | 0.16207 |
Rwork | 0.160 |
R-free | 0.19395 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ib0 |
RMSD bond length | 0.028 |
RMSD bond angle | 2.693 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.200 | 1.490 |
High resolution limit [Å] | 1.460 | 1.460 |
Number of reflections | 66074 | |
<I/σ(I)> | 27.9 | 2 |
Completeness [%] | 99.4 | 97.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.01M Znso4, 0.1M MES, 25% PEG, Monomethyl Ether 550 , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |